Hydrogen-rich PBS protects cultured human cells from ionizing radiation-induced cellular damage

Abstract

Hydroxyl radicals play an important role in ionizing radiation-induced cellular damage, while hydrogen can selectively reduce hydroxyl radicals in vitro. This study was designed to test the hypothesis that hydrogen-rich PBS may be an effective radioprotective agent in vitro. Com pared to cells pretreated with out hydrogen, we demonstrated that treating cells with hydrogen-rich PBS be fore irradiation could significantly inhibit IR-induced apoptosis, increase viability of human intestinal crypt cells, significantly increase endogenous antioxidant, and decrease malondialdehyde and 8-hydroxydeoxyguanosine concentrations of human lymphocyte AHH-1 cells. It is concluded that hydrogen has a potential as an effective and safe radioprotective agent.

Study details

Article type

Original Research

Disease / injury model

Ionizing radiation-induced cellular damage (in vitro model)

Dose comparison

Different concentrations of hydrogen-rich PBS (0.1-0.4 mmol/L) were tested for radioprotective effects.

Dose-dependent effect

Yes

Outcome

Positive

Findings

Hydrogen-rich PBS significantly protected cultured human cells from ionizing radiation-induced damage by inhibiting apoptosis, increasing cell viability, enhancing endogenous antioxidant status, and reducing markers of oxidative stress and DNA damage.