In VitroHighlightedHeme oxygenase-1 mediates the anti-inflammatory effect of molecular hydrogen in LPS-stimulated RAW 264.7 macrophagesMHID-1519950PMID: 241487942013International Journal of SurgeryHong-Guang Chen, Ke-Liang Xie, Huan-Zhi Han, Wei-Na Wang, Da-Quan Liu, Guo-Lin Wang, et al.Background: Molecular hydrogen (H2) as a new medical gas has an anti-inflammatory effect. In the present study, we investigated whether heme oxygenase-1 (HO-1) contributes to the anti-inflammatory effect of H2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: RAW 264.7 macrophages were stimulated by LPS (1 mg/mL) with presence or absence of different concentrations of H2. Cell