Clinical TrialAnimal StudyIn VitroHighlighted
MHID-2649268PMID: 357349742022Journal of Cellular and Molecular Medicine
Geru Tao, Guangjie Zhang, Wei Chen, Chao Yang, Yazhuo Xue, Guohua Song, et al.
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease
worldwide with increasing incidence consistent with obesity, type 2 diabetes and
cardiovascular diseases. No approved medication was currently available for NAFLD
treatment. Molecular hydrogen (H2), an anti-oxidative, anti-inflammatory biomedical
agent is proved to exhibit therapeutic and preventive effect in variou
In VitroHighlighted
MHID-6868466PMID: 352700302022International Journal of Molecular Sciences
Tomoya Hasegawa, Mikako Ito, Satoru Hasegawa, Masaki Teranishi, Koki Takeda, Shuto Negishi, et al.
Molecular hydrogen ameliorates pathological states in a variety of human diseases, animal models, and cell models, but the effects of hydrogen on cancer have been rarely reported. In addition, the molecular mechanisms underlying the effects of hydrogen remain mostly unelucidated. We found that hydrogen enhances proliferation of four out of seven human cancer cell lines (the responders). The prolif
Animal StudyHighlighted
MHID-2655528PMID: 352732492022Scientific Reports
Yao Mawulikplimi Adzavon, Fei Xie, Yang Yi, Xue Jiang, Xiaokang Zhang, Jin He, et al.
Molecular hydrogen (H2) has emerged as a new therapeutic option in several diseases and is widely adopted by healthy people. However, molecular data to support therapeutic functions attributed to the biological activities of H2 remain elusive. Here, using transcriptomic and metabolomic approaches coupled with biochemistry and micro-CT technics, we evaluated the effect of long-term (6 months) and d
Animal StudyIn VitroHighlighted
MHID-5758484PMID: 353001732022Oxidative Medicine and Cellular Longevity
Yujie Li, Chengcheng Shen, Xin Zhou, Jianghe Zhang, Xiaoyue Lai, Yiming Zhang
Wound healing is a complex dynamic process involving a large number of biological events. Excessive oxidative stress is a key factor delaying wound healing. Hydrogen is an antioxidant, anti-inflammatory, and antiapoptotic medical gas with safety, effectiveness, and penetrability. However, the effects of local treatment of hydrogen on wound healing and its potential mechanisms remain unclear. In this
In Vitro
MHID-1605109PMID: 345336462022Molecular and Cellular Biochemistry
Yasukazu Saitoh, Nene Yonekura, Daigo Matsuoka, Akira Matsumoto
Periodontitis is defined as a multifactorial polymicrobial infection accompanied by inflammatory reactions. Porphyromonas gingivalis (Pg) is known as a major pathogen in the initiation and progression of periodontitis, and a major virulence factor is Pg lipopolysaccharide (LPS). Molecular hydrogen (H2) has been reported to act as a gaseous antioxidant, which suppresses periodontitis progression by
In VitroHighlighted
MHID-0634713PMID: 347707842021Molecules
In-Soo You, Subham Sharma, Ailyn Fadriquela, Johny Bajgai, Thuy Trinh Thi, Md. Habibur Rahman, et al.
Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H2) gas has been explored for its antioxidant properties. This study investigated the role of H2 gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) in vitro. Results show that tim
In VitroOtherHighlighted
MHID-32438852021Journal of Plant Growth Regulation
Xin Zhang, Fei Xie, Zhao Zhang, Yao Mawulikplimi Adzavon, Zehua Su, Qinghui Zhao, et al.
Molecular hydrogen has been shown to exert beneficial effects on plant growth and abiotic stress tolerance, however, the exact mechanism has not yet been fully elucidated. H2 is able to diffuse directly across the plasma membrane, which is the first barrier between H2 and cells. It is possible that H2 may exert its biological effects on plant cells by interacting with PM. Hydrogenases can catalyze
In VitroHighlighted
MHID-0456102PMID: 347832272021Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)
Jinfang LU, Jin ZHENG, Yadi WANG, Jie CHENG, Xueling LI, Jun HU, et al.
Hydrogen (H2)-rich water, an apparent source of molecular H2, is an emerging functional drink with many purported benefits for human health (Yang et al., 2020; Ostojic, 2021). The preventive and therapeutic effects of H2 on various pathological processes have been intensively investigated in numerous clinical trials; it is commonly believed that the beneficial effects are mainly attributed to its
Clinical TrialIn VitroHighlighted
MHID-7483898PMID: 338995412021Experimental Biology and Medicine
Yanhong Si, Hua Tian, Bingqing Dong, Ying Zhang, Yuanyuan Wen, Xiubin Jia, et al.
Oxidative stress and inflammation are closely related to atherosclerotic cardiovascular disease. It is established that hydrogen has significant protective effects on many diseases as a potential antioxidative and anti-inflammatory agent. The purpose of this study is to evaluate the effect of hydrogen on unstable angina in vitro and in vivo. An atherosclerosis model in vitro was constructed by ox-
In Vitro
MHID-0313161PMID: 340286462021Molecular and Cellular Biochemistry
Yasukazu Saitoh, Yuuki Yamaguchi, Yuhei Okada
Molecular hydrogen (H2) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. However, there are still many unclear points regarding its effectiveness in the skin. Therefore, the purpose of this study was to examine the protective effect of H2 against ultraviolet (UV) irradiation-related stress injury in human epide
In VitroHighlighted
MHID-35801392020Molecular & Cellular Toxicology
Rahima Begum, Cheol‑Su Kim, Ailyn Fadriquela, Johny Bajgai, Xingyu Jing, Dong‑Heui Kim, et al.
Background: Oxidative stress is involved in the development of many inflammatory, metabolic and aging diseases.
Objective: In this study we investigated, the protective effects of H2 on RAW 264.7 macrophage cell against LPS-and H2O2-induced oxidative stress by the inhibition of MAPK pathway and also activate the Nrf2 pathway.
Results: Our results showed H2 increased the macrophage cell prolifera
In VitroEx VivoHighlighted
MHID-98806872020bioRxiv
Xin Zhang, Zhao Zhang, Zehua Su, Qinghui Zhao, Tyler W. LeBaron, Qinjian Li, et al.
Molecular hydrogen (H2) exhibits a wide breadth of botanical effects especially in improving the resistance to abiotic stress. However, the primary target is not clear. Bidirectional hydrogenases have been well studied in microbes and alga, but there is a paucity of research in higher plants. Here, we used real-time spectrophotometry, gas chromatography and H2 sensor to detect the H2-evolving and
In VitroHighlighted
MHID-7135279PMID: 345586532020Journal of Investigative Dermatology
Wei Fang, Luyan Tang, Guizhen Wang, Jinran Lin, Wanqing Liao, Weihua Pan, et al.
Oxidative stress is proven to be critical for the initiation and progression of vitiligo. Molecular hydrogen (H2) possesses potent antioxidant activity and has been shown to protect against various oxidative stresserelated diseases. In this study, we first investigated the effects and mechanisms of H2 in human melanocytes damaged by hydrogen peroxide. We initially found that H2 reduced intracellula
In VitroIn SilicoHighlighted
MHID-67149392018PLoS ONE
Anzu Suzuki, Mikako Ito, Tomonori Hamaguchi, Hiroshi Mori, Yuka Takeda, Ryuko Baba, et al.
Oral administration of hydrogen water ameliorates Parkinson’s disease (PD) in rats, mice, and humans. We previously reported that the number of putative hydrogen-producing bacte- ria in intestinal microbiota is low in PD compared to controls. We also reported that the amount of hydrogen produced by ingestion of lactulose is low in PD patients. The decreased hydrogen production by intestinal microb
Animal StudyIn VitroHighlighted
MHID-18249022018Translational Cancer Research
Lei Shang, Fei Xie, Jiala Li, Yating Zhang, Mengyu Liu, Pengxiang Zhao, et al.
Background: To investigate the potential effects of molecular hydrogen on ovarian cancer.
Methods: The in vivo study was performed using Hs38.T xenografted BALB/c nude mice. The mean tumor volume was monitored during 6 weeks of hydrogen inhalation. Immunohistochemistry (IHC) staining was performed to determine the Ki67 and CD34 expression. The in vitro effects of molecular hydrogen on proliferati
Animal StudyIn VitroHighlighted
MHID-4778348PMID: 303151732018Nature Communications
Penghe Zhao, Zhaokui Jin, Qian Chen, Tian Yang, Danyang Chen, Jin Meng, et al.
By delivering the concept of clean hydrogen energy and green catalysis to the biomedical field, engineering of hydrogen-generating nanomaterials for treatment of major diseases holds great promise. Leveraging virtue of versatile abilities of Pd hydride nanomaterials in high/stable hydrogen storage, self-catalytic hydrogenation, near-infrared (NIR) light absorption and photothermal conversion, here
In VitroHighlighted
MHID-1525156PMID: 299011392018Molecular Medicine Reports
YU JIANG, GANG LIU, LI ZHANG, SHENG CHENG, CHUN LUO, YANG LIAO, et al.
The aim of the present study was to investigate the effects of combination therapy of LY294002, a specific inhibitor of phosphatidylinositol 3‑kinase (PI3K), with hydrogen‑rich saline on the proliferation and apoptosis of the non‑small cell lung cancer (NSCLC) A549 cell line and the mechanisms underpinning this. Excessive production of reactive oxygen species (ROS) may induce DNA mutations, DNA d
Ex VivoHighlighted
MHID-8457494PMID: 283509672017Canadian Journal of Physiology and Pharmacology
Marek Zálešák, Branislav Kura, Ján Graban, Veronika Ledvényiová-Farkašová, Jan Slezak, Tatiana Ravingerova
Generation of free radicals through incomplete reduction of oxygen during ischemia/ reperfusion is well-described. On the other hand, molecular hydrogen (H2) reduces oxidative stress due to its ability to react with strong oxidants and easily penetrate cells by diffusion, without disturbing metabolic redox reactions. This study was designed to explore cardioprotective potential of hypoxic postcond
In VitroHighlighted
MHID-1014265PMID: 286201982017Scientific Reports
Ching-Ying Wu, Wen-Li Hsu, Ming-Hsien Tsai, Jui-Lin Liang, Jian-He Lu, Chia-Jung Yen, et al.
Based on the oxidative stress theory, aging derives from the accumulation of oxidized proteins induced by reactive oxygen species (ROS) in the cytoplasm. Hydrogen peroxide (H2O2) elicits ROS that induces skin aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl groups. Decreased Ca2+ signaling is observed in aged cells, probably secondary to the for
In Vitro
MHID-7973359PMID: 284674972017PLOS ONE
Yayoi Murakami, Masafumi Ito, Ikuroh Ohsawa
Inhalation of molecular hydrogen (H2) gas ameliorates oxidative stress-induced acute injuries in the brain. Consumption of water nearly saturated with H2 also prevents chronic neurodegenerative diseases including Parkinson’s disease in animal and clinical studies. However, the molecular mechanisms underlying the remarkable effect of a small amount of H2 remain unclear. Here, we investigated the ef
