Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury

Abstract

Objective. To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury. Methods. 36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (𝑛= 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-rich saline treatment group (I+HS). 30 min after injury, normal saline and hydrogen-rich saline were injected intraperitoneally (5 mL/kg) in I+NS group and I+HS group, respectively. All rats were euthanized and blood and organ specimens were collected for determination 24 h after inhalation injury. Results. Tumor necrosis factor-alpha (TNF-𝛼) levels, malondialdehyde (MDA) concentrations, nuclear factor kappa B (NF-𝜅B) p65 expression, and apoptosis index (AI) in I+HS group were significantly decreased (𝑃< 0.05), while superoxide dismutase (SOD) activities were increased compared with those in I+NS group; and a marked improvement in alveolar structure was also found after hydrogen-rich saline treatment. Conclusions. Hydrogen-rich saline treatment exerts protective effects in acute lung injury induced by inhalation injury, at least in part through the activation of anti-inflammatory and antioxidant pathways and inhibition of apoptosis.

Study details

Article type

Original Research

Disease / injury model

Acute lung injury (ALI) induced by smoke inhalation

Duration

24 hours

Dose comparison

Only one dose/concentration of hydrogen-rich saline was used (5 mL/kg, 0.6-0.79 mmol/L).

Outcome

Positive

Findings

Hydrogen-rich saline significantly ameliorated lung injury caused by smoke inhalation in rats, reducing inflammation, oxidative stress, and apoptosis, and improving lung structure.