OXIDATIVE MODIFICATION OF PROTEINS IN COLORECTAL CANCER UNDER THE INFLUENCE OF 5-FLUOROURACIL AND MOLECULAR HYDROGEN

Abstract

The aim of the study – to investigate the eff ect of water saturated with molecular hydrogen and 5-fl uorouracil on the content of carbonyl groups of oxidatively modifi ed proteins in the blood serum of white rats with colorectal cancer. Material and Methods. The experiments were conducted on 50 male Wistar rats. The animals were modeled with colorectal cancer (CRC) by subcutaneous injection of 1,2-dimethylhydrazine (DMH) at a dose of 7.2 mg/kg body weight once a week for 30 weeks. 5-Fluorouracil was administered intraperitoneally for 4 days at 12 mg/kg and for another 4 days every other day at 6 mg/kg. Animals consumed water enriched with molecular hydrogen at a concentration of 0.6 ppm ad libitum. Rats were euthanized under thiopental anesthesia. Blood serum was used for the study, in which the content of carbonyl groups was determined by the colorimetric method. Statistical data processing was performed using the SPSS-22 software package. Results. It was found that the modeling of colorectal cancer led to a 1.77-fold increase in the content of carbonyl groups in the blood serum of rats compared to intact animals. The content of carbonyl groups in the blood serum of rats with CRC treated with chemotherapy by administration of 5-fl uorouracil increased 1.93 times compared to the intact group and 1.1 times compared to animals with CRC. Consumption of water saturated with molecular hydrogen 30 days after modeling CRC and administration of 5-fl uorouracil to white rats led to a 1.18-fold decrease in the content of carbonyl groups in their blood serum compared to animals with CRC who were administered 5-fl uorouracil but did not consume water saturated with molecular hydrogen. Conclusions. The use of molecular hydrogen saturated water is an eff ective method of reducing oxidative modifi cation of proteins in rats with colorectal cancer under 5-fl uorouracil chemotherapy.