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In VitroHighlighted

Hydrogen gas improves proliferation and mitochondrial activity of human adipose-derived stem cells after cryopreservation

MHID-5509595PMID: 392466992024Regenerative Therapy

Koji Kimura, Yasuhiko Tabata

The objective of this study is to evaluate the effect of hydrogen gas on the biological functions of human adipose-derived stem cells (hADSC) in cryopreservation. hADSC were cryopreserved by a commercial cell preservation solution in the presence of hydrogen gas. After cryopreservation at 80 C, the viability, initial attachment morphology, and biological parameters of cells cryopreserved were eval

In Vitro

Protective effects of dissolved molecular hydrogen against hydrogen peroxide‑, hydroperoxide‑, and glyoxal‑induced injuries to human skin keratinocytes

MHID-0313161PMID: 340286462021Molecular and Cellular Biochemistry

Yasukazu Saitoh, Yuuki Yamaguchi, Yuhei Okada

Molecular hydrogen (H2) is recognized as a gaseous antioxidant, and it is expected to ameliorate various disorders related to oxidative stress and inflammation. However, there are still many unclear points regarding its effectiveness in the skin. Therefore, the purpose of this study was to examine the protective effect of H2 against ultraviolet (UV) irradiation-related stress injury in human epide

In VitroObservational Study

Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

MHID-9621518PMID: 220709002011Journal of Photochemistry and Photobiology B: Biology

Shinya Kato, Yasukazu Saitoh, Keizou Iwai, Nobuhiko Miwa

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41ºC and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and +184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold m