Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

Abstract

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41ºC and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and +184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold more abundantly by HW application for 3-5 days than RW in OUMS-36 fibroblasts, and localized preferentially around the nuclei as shown by immunostain. HW application significantly prevented cell death and DNA damages such as nuclear condensation and fragmentation in UVA-induced HaCaT keratinocytes as estimated by WST-1 and Hoechst33342 assays. HW significantly suppressed UVA-induced generation of intracellular superoxide anion radicals in both the cell lines according to NBT assay. Wrinkle repression was clinically assessed using a HW-bathing. Six Japanese subjects were enrolled in a trial of HW-bathing (DH, 0.2-0.4 ppm) every day for 3 months. HW-bathing significantly improved wrinkle in four subjects on the back of neck on 90th day as compared to 0 day. Thus, HW may serve as daily skin care to repress UVA-induced skin damages by ROS-scavenging and promotion of type-I collagen synthesis in dermis. Keywords: hydrogen water, UV-A, collagen type-I, fluorescent immunostaining, oxidative stress, hydrogen water bathing, wrinkle repression

Study details

Article type

Original Research

Disease / injury model

UVA-induced skin damage, wrinkle formation

Duration

3 months

Dose-dependent effect

No

Outcome

Positive

Findings

Hydrogen-rich electrolyzed warm water (HW) promoted type-I collagen synthesis in fibroblasts, suppressed UVA-induced cell death and DNA damage in keratinocytes, reduced intracellular ROS, and clinically improved wrinkles in human subjects after 3 months of daily HW-bathing.