In VitroHighlighted
MHID-0389345PMID: 415753722026Biochemical Journal
Sabrina Dezzani, Abdulrahman Alogaidi, Anca Pordea, Simone Morra
The production of biofuels by bacterial fermentation receives sustained attention due to the need to develop novel circular and sustainable technologies. Clostridium beijerinckii produces both hydrogen (H2) and carbon-based biofuels acetone, butanol and ethanol (ABE solvents). H2 metabolism in C. beijerinckii is complex and mostly unexplored. Seven hydrogenase genes are contained in the genome, bu
Literature ReviewHighlighted
MHID-2922681PMID: 413737252025International Journal of Molecular Sciences
Daria Kornieieva, Barbora Kalocayova, Jan Slezak, Branislav Kura
Heart failure (HF) is increasing in prevalence in many countries around the world. HF is a complex clinical syndrome characterized by the heart’s inability to pump blood effectively, resulting in significant morbidity and mortality. After an initial cardiac event (e.g., myocardial infarction, valve dysfunction, hypertension, etc.), adaptive mechanisms are activated to preserve cardiac function. Su
Animal StudyEx VivoHighlighted
MHID-4421003PMID: 398164752025Bioactive Materials
Yuanming An, Haozhi Zhang, Shi’an Zhang, Yuantao Zhang, Lizhen Zheng, Xin Chen, et al.
Biodegradable magnesium (Mg) implant generally provides temporary fracture fixation and facilitates bone regeneration. However, the exact effects of generated Mg ions (Mg2+), hydrogen gas (H2), and hydroxide ions (OH-) by Mg degradation on enhancing fracture healing are not fully understood. Here we investigate the in vivo degradation of Mg intramedullary nail (Mg-IMN), revealing the generation of
In SilicoHighlighted
MHID-6447318PMID: 410089612025Antioxidants
Sumaiya Akhter Ria, Jintana Meesungnoen, Jean-Paul Jay-Gerin
(1) Background: Water, comprising about 70–80% of cellular mass, is the most abundant constituent of living cells. Upon exposure to ionizing radiation, water undergoes radiolysis, generating a variety of reactive species, including free radicals and molecular products. Among these, hydroxyl radicals (•OH) are particularly damaging due to their very high reactivity and their capacity to induce oxid
Animal StudyIn VitroHighlighted
MHID-8939519PMID: 413302172025Redox Biology
Shuto Negishi, Mikako Ito, Tomoya Hasegawa, Hikaru Otake, Bisei Ohkawara, Akio Masuda, et al.
The mechanisms underlying the biomedical effects of molecular hydrogen (H2) remain poorly understood and
are often attributed to its selective reduction of hydroxyl radicals, based on the long-held notion that H2 is
biologically inert. We demonstrate that H2 is biologically active, specifically targeting the Rieske iron-sulfur
protein (RISP). We first observed that H2 induces the mitochondrial unf
Literature ReviewHighlighted
MHID-44233202024Molecular Hydrogen in Health and Disease, Advances in Biochemistry in Health and Disease
Shin-ichi Hirano, Yusuke Ichikawa, Bunpei Sato, Yoshiyasu Takefuji, Xiao-Kang Li, Fumitake Satoh
Mitochondria originated from aerobic bacteria in endosymbiosis. Through this symbiosis, eukaryotes acquired an efficient energy-producing system, but at the cost of exposure to oxidative stress from reactive oxygen species (ROS). Molecular hydrogen (H2) was recently identified as an antioxidant that selectively reduces ROS, such as hydroxyl radicals and peroxynitrite, which are strong oxidants, and
Literature ReviewHighlighted
MHID-1339888PMID: 397659102024Antioxidants
Mikhail Yu. Artamonov, Felix A. Pyatakovich, Inessa A. Minenko
In regenerative medicine, mesenchymal stem cells (MSCs) have shown their importance and potential in tissue reconstruction and immune system modification. However, such cells’ potential is often diminished by factors such as oxidative stress, immune rejection, and inadequate engraftment. This review highlights the role of molecular hydrogen (H2) and cold atmospheric plasma (CAP) as adjunct therapi
Mechanisms ReviewHighlighted
MHID-3162417PMID: 386754292024Pharmaceuticals
Mikhail Yu. Artamonov, Tyler W. LeBaron, Felix A. Pyatakovich, Inessa A. Minenko
Stem cell therapy has emerged as a promising avenue for regenerative medicine, offering the potential to treat a wide range of debilitating diseases and injuries. Among the various types of stem cells, mesenchymal stem cells (MSCs) have garnered significant attention due to their unique properties and therapeutic potential. In recent years, researchers have been exploring novel approaches to enhan
In VitroHighlighted
MHID-5509595PMID: 392466992024Regenerative Therapy
Koji Kimura, Yasuhiko Tabata
The objective of this study is to evaluate the effect of hydrogen gas on the biological functions of human adipose-derived stem cells (hADSC) in cryopreservation. hADSC were cryopreserved by a commercial cell preservation solution in the presence of hydrogen gas. After cryopreservation at 80 C, the viability, initial attachment morphology, and biological parameters of cells cryopreserved were eval
Systematic ReviewHighlighted
MHID-3427886PMID: 373542432023Asian Pacific Journal of Cancer Prevention
Muhammad Nooraiman Zufayri Mohd Noor, Adlin Sofea Alauddin, Yin How Wong, Chung Yeng Looi, Eng Hwa Wong, Priya Madhavan, et al.
Background: Cancer remains a challenging target to cure, with present therapeutic methods unable to exhibit restorative outcomes without causing severe negative effects. Molecular hydrogen (H2) has been reported to be a promising adjunctive therapy for cancer treatment, having the capability to induce anti-proliferative, anti-oxidative, pro-apoptotic and anti-tumoural effects. This review summaris
Clinical TrialIn VitroObservational StudyHighlighted
MHID-30414712023Processes
Richard J. Bloomer, Yufeng Zhang, Joshua Y. Shirazi, Chidimma Okegbe, Jacquelyn Pence, Keith Martin, et al.
Alpha Hope is a dietary supplement containing pyrroloquinoline quinone and elemental magnesium, which produces molecular hydrogen (H2 gas) when dissolved in water. We determined the impact of Alpha Hope on Nrf-2, peroxisome proliferator-activated receptor-gamma coactivator (PGC-1α), and citrate synthase (CS) activity in C2C12 myoblasts. Alpha Hope was titrated to three concentrations of pyrroloqui
In VitroHighlighted
MHID-35766222022Processes
Subham Sharma, Johny Bajgai, Jayson M. Antonio, Ailyn Fadriquela, Thuy Thi Trinh, Md. Habibur Rahman, et al.
Diabetes is coupled with hyperglycemia, a state in which elevated glucose levels trigger oxidative stress (OS) in various body functions. One of the organs most afflicted by diabetes is the kidney. Despite this, specific treatments to mitigate the harmful effects of hyperglycemia-induced OS in the kidney have not been extensively explored. This study evaluates the anti-hyperglycemic efficacy of magne
Animal StudyIn VitroHighlighted
MHID-2681523PMID: 355281642022BioMed Research International
Xiangyan Zhang, Geru Tao, Ya’nan Zhao, Sijie Xing, Jie Jiang, Boyan Liu, et al.
Objective. Molecular hydrogen (H2) has been considered a potential therapeutic target in many cancers. Therefore, we sought to assess the potential effect of H2 on colorectal cancer (CRC) in this study.
Methods. The effect of H2 on the proliferation and apoptosis of RKO, SW480, and HCT116 CRC cell lines was assayed by CCK-8, colony formation, and flow cytometry assays. The effect of H2 on tumor growt
In VitroHighlighted
MHID-6868466PMID: 352700302022International Journal of Molecular Sciences
Tomoya Hasegawa, Mikako Ito, Satoru Hasegawa, Masaki Teranishi, Koki Takeda, Shuto Negishi, et al.
Molecular hydrogen ameliorates pathological states in a variety of human diseases, animal models, and cell models, but the effects of hydrogen on cancer have been rarely reported. In addition, the molecular mechanisms underlying the effects of hydrogen remain mostly unelucidated. We found that hydrogen enhances proliferation of four out of seven human cancer cell lines (the responders). The prolif
Animal StudyHighlighted
MHID-2655528PMID: 352732492022Scientific Reports
Yao Mawulikplimi Adzavon, Fei Xie, Yang Yi, Xue Jiang, Xiaokang Zhang, Jin He, et al.
Molecular hydrogen (H2) has emerged as a new therapeutic option in several diseases and is widely adopted by healthy people. However, molecular data to support therapeutic functions attributed to the biological activities of H2 remain elusive. Here, using transcriptomic and metabolomic approaches coupled with biochemistry and micro-CT technics, we evaluated the effect of long-term (6 months) and d
Animal StudyIn VitroHighlighted
MHID-5758484PMID: 353001732022Oxidative Medicine and Cellular Longevity
Yujie Li, Chengcheng Shen, Xin Zhou, Jianghe Zhang, Xiaoyue Lai, Yiming Zhang
Wound healing is a complex dynamic process involving a large number of biological events. Excessive oxidative stress is a key factor delaying wound healing. Hydrogen is an antioxidant, anti-inflammatory, and antiapoptotic medical gas with safety, effectiveness, and penetrability. However, the effects of local treatment of hydrogen on wound healing and its potential mechanisms remain unclear. In this
In VitroHighlighted
MHID-0634713PMID: 347707842021Molecules
In-Soo You, Subham Sharma, Ailyn Fadriquela, Johny Bajgai, Thuy Trinh Thi, Md. Habibur Rahman, et al.
Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H2) gas has been explored for its antioxidant properties. This study investigated the role of H2 gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) in vitro. Results show that tim
In VitroOtherHighlighted
MHID-32438852021Journal of Plant Growth Regulation
Xin Zhang, Fei Xie, Zhao Zhang, Yao Mawulikplimi Adzavon, Zehua Su, Qinghui Zhao, et al.
Molecular hydrogen has been shown to exert beneficial effects on plant growth and abiotic stress tolerance, however, the exact mechanism has not yet been fully elucidated. H2 is able to diffuse directly across the plasma membrane, which is the first barrier between H2 and cells. It is possible that H2 may exert its biological effects on plant cells by interacting with PM. Hydrogenases can catalyze
In VitroHighlighted
MHID-0456102PMID: 347832272021Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)
Jinfang LU, Jin ZHENG, Yadi WANG, Jie CHENG, Xueling LI, Jun HU, et al.
Hydrogen (H2)-rich water, an apparent source of molecular H2, is an emerging functional drink with many purported benefits for human health (Yang et al., 2020; Ostojic, 2021). The preventive and therapeutic effects of H2 on various pathological processes have been intensively investigated in numerous clinical trials; it is commonly believed that the beneficial effects are mainly attributed to its
In VitroHighlighted
MHID-35801392020Molecular & Cellular Toxicology
Rahima Begum, Cheol‑Su Kim, Ailyn Fadriquela, Johny Bajgai, Xingyu Jing, Dong‑Heui Kim, et al.
Background: Oxidative stress is involved in the development of many inflammatory, metabolic and aging diseases.
Objective: In this study we investigated, the protective effects of H2 on RAW 264.7 macrophage cell against LPS-and H2O2-induced oxidative stress by the inhibition of MAPK pathway and also activate the Nrf2 pathway.
Results: Our results showed H2 increased the macrophage cell prolifera
