Animal StudyIn VitroHighlighted
MHID-7372149PMID: 268455012016
Yukako Muramatsu, MD, Mikako Ito, PhD, Takahiro Oshima, Seiji Kojima, MD, PhD, Kinji Ohno, MD, PhD
Bronchopulmonary dysplasia (BPD) is characterized by developmental arrest of the alveolar tissue. Oxidative stress is causally associated with development of BPD. The effects of hydrogen have been reported in a wide range of disease models and human diseases especially caused by oxidative stress. We made a rat model of BPD by injecting lipopolysaccharide (LPS) into the amniotic fluid at E16.5. The
Animal StudyIn VitroHighlighted
MHID-8944005PMID: 303740532016Scientific Reports
Yingni Lin, Bisei Ohkawara, Mikako Ito, Nobuaki Misawa, Kentaro Miyamoto, Yasuhiko Takegami, et al.
Molecular hydrogen (H2) is effective for many diseases. However, molecular bases of H2 have not been fully elucidated. Cumulative evidence indicates that H2 acts as a gaseous signal modulator. We found that H2 suppresses activated Wnt/β-catenin signaling by promoting phosphorylation and degradation οf β-catenin. Either complete inhibition of GSK3 or mutations at CK1- and GSK3-phosphorylation sites
Animal StudyHighlighted
MHID-6432478PMID: 259258892015BMC Neuroscience
Satoru Takeuchi, Kimihiro Nagatani, Naoki Otani, Hiroshi Nawashiro, Takashi Sugawara, Kojiro Wada, et al.
Background: Enhanced oxidative stress occurs in spontaneously hypertensive stroke-prone rats (SHRSP), and is important in blood–brain barrier (BBB) disruption. Hydrogen can exert potent protective cellular effects via reduction in oxidative stress in various diseases. The present study investigated whether long-term hydrogen treatment can improve neurological function outcome in the SHRSP model, a
Animal StudyHighlighted
MHID-0865356PMID: 213757532015The Journal of Thoracic and Cardiovascular Surgery
Yasuaki Kishimoto, MD, Taichi Kato, MD, PhD, Mikako Ito, PhD, Yoshiteru Azuma, MD, Yoshie Fukasawa, MD, Kinji Ohno, MD, PhD, et al.
Objective: The pathogenesis of pulmonary arterial hypertension (PAH) involves reactive oxygen species and inflammation. Beneficial effects of molecular hydrogen, which exerts both anti-inflammatory and antioxidative effects, have been reported for various pathologic conditions. We therefore hypothesized that molecular hydrogen would improve monocrotaline (MCT)-induced PAH in rats.
Methods: Nineteen
Literature ReviewHighlighted
MHID-7479916PMID: 264839532015Medical Gas Research
Masatoshi Ichihara, Sayaka Sobue, Mikako Ito, Masafumi Ito, Masaaki Hirayama, Kinji Ohno
Therapeutic effects of molecular hydrogen for a wide range of disease models and human diseases have been investigated since 2007. A total of 321 original articles have been published from 2007 to June 2015. Most studies have been conducted in Japan, China, and the USA. About three-quarters of the articles show the effects in mice and rats. The number of clinical trials is increasing every year. I
Animal StudyHighlighted
MHID-4053251PMID: 260900702015Oxidative Medicine and Cellular Longevity
Xing Chen, Qi Liu, Dawei Wang, Shihai Feng, Yongjian Zhao, Yun Shi, et al.
Objective. To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury.
Methods. 36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (𝑛= 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-rich saline treatment group (I+HS). 30 min after injury, normal saline and hydr
Animal StudyHighlighted
MHID-8429491PMID: 259549912015Molecular Medicine Reports
HONGWEI LIU, NING HUA, Keliang Xie, TINGTING ZHAO, Yonghao Yu
Overactivation of poly (ADP-ribose) polymerase 1 (PARP-1), as a result of sustained DNA oxidation in ischemia‑reperfusion injury, triggers programmed cell necrosis and apoptosis. The present study was conducted to demonstrate whether hydrogen‑rich saline (HRS) has a neuroprotective effect on retinal ischemia reperfusion (RIR) injury through inhibition of PARP‑1 activation. RIR was induced by trans
Animal StudyHighlighted
MHID-9762050PMID: 266686282015Experimental and Therapeutic Medicine
Xiaoyin Meng, Hongguang Chen, Guolin Wang, Yonghao Yu, Keliang Xie
Hydrogen has been reported to exert a therapeutic effect in several diseases due to its antioxidative, anti‑inflammatory and anti‑apoptotic properties. The aim of the present study was to investigate whether hydrogen‑rich saline treatment could attenuate ovarian damage induced by cisplatin. A total of 240 adult, virgin, female Sprague Dawley rats, weighing 180‑220 g, were randomly divided into fou
Animal StudyHighlighted
MHID-5889693PMID: 257075802015Molecular and Cellular Biochemistry
Sayaka Sobue, Kazuaki Yamai, Mikako Ito, Kinji Ohno, Masafumi Ito, Takashi Iwamoto, et al.
Molecular hydrogen (H2) is an agent with po- tential applications in oxidative stress-related and/or in- flammatory disorders. H2 is usually administered by inhaling H2-containing air (HCA) or by oral intake of H2- rich water (HRW). Despite mounting evidence, the mole- cular mechanism underlying the therapeutic effects and the optimal method of H2 administration remain unclear. Here, we investigate
Animal StudyHighlighted
MHID-4748086PMID: 260479402015Journal of Translational Medicine
Song‑Xue Guo, Quan Fang, Chuan‑Gang You, Yun‑Yun Jin, Xin‑Gang Wang, Xin‑Lei Hu, et al.
Background: Early acute kidney injury (AKI) in severely burned patients predicts a high mortality that is multi-factorial. Hydrogen has been reported to alleviate organ injury via selective quenching of reactive oxygen species. This study investigated the potential protective effects of hydrogen against severe burn-induced early AKI in rats.
Methods: Severe burn were induced via immersing the sha
Animal StudyIn VitroHighlighted
MHID-0826156PMID: 246851142014CNS Neuroscience & Therapeutics
Fang-Ting Liu, Sheng-Ming Xu, Zheng-Hua Xiang, Xiang-Nan Li, Jian Li, Hong-Bin Yuan, et al.
Aims: Spinal cord injury (SCI) can induce excessive astrocyte activation. Hydrogen has been deemed as a novel antioxidant. We investigated whether molecular hydrogen could act as an antiastrogliosis agent during SCI and oxidative injury in experimental rats and cultured astrocytes. Methods: Hydrogen-rich saline (HS, 8 mL/kg, i.p.) was injected every 12 h after SCI in rats. The expression of STAT3,
Animal StudyHighlighted
MHID-4606624PMID: 245091622014Free Radical Biology and Medicine
Yukio Mano, Tomomi Kotani, Mikako Ito, Taku Nagai, Yuko Ichinohashi, Kiyofumi Yamada, et al.
Molecular hydrogen (H2) scavenges hydroxyl radicals. Recently, H2 has been reported to prevent a variety of diseases associated with oxidative stress in model systems and in humans. Here, we studied the effects of H2 on rat fetal hippocampal damage caused by ischemia and reperfusion (IR) on day 16 of pregnancy with the transient occlusion of the bilateral utero-ovarian arteries. Starting 2 days be
Animal StudyHighlighted
MHID-61354972014Scientific Reports
Chi Liu, Ryosuke Kurokawa, Masayuki Fujino, Shinichi Hirano, Bunpei Sato, Xiao-Kang Li
Hydrogen exerts beneficial effects in disease animal models of ischemia-reperfusion injury as well as inflammatory and neurological disease. Additionally, molecular hydrogen is useful for various novel medical and therapeutic applications in the clinical setting. In the present study, the hydrogen concentration in rat blood and tissue was estimated. Wistar rats were orally administered hydrogen su
Animal StudyHighlighted
MHID-1417416PMID: 234757672013American Journal of Physiology - Lung Cellular and Molecular Physiology
Tomohiro Kawamura, Nobunao Wakabayashi, Norihisa Shigemura, Chien-Sheng Huang, Kosuke Masutani, Yugo Tanaka, et al.
Hyperoxic lung injury is a major concern in critically ill patients who receive high concentrations of oxygen to treat lung diseases. Successful abrogation of hyperoxic lung injury would have a huge impact on respiratory and critical care medicine. Hydrogen can be administered as a therapeutic medical gas. We recently demonstrated that inhaled hydrogen reduced transplant-induced lung injury and in
Animal StudyHighlighted
MHID-3846502PMID: 359044492013Molecular Vision
Chun-xiao Yang, Hong Yan, Tian-bing Ding
Purpose: The aim of this study was to investigate the potential antioxidative effect and mechanism for the protective effects of hydrogen saline on selenite-induced cataract in rats.
Methods: Sprague-Dawley rat pups were divided into the following groups: control (Group A), selenite induced (Group B), and selenite plus hydrogen saline treated (Group C). Rat pups in Groups B and C received a singl
Animal StudyIn VitroHighlighted
MHID-7737580PMID: 231402242012Transplant International
Kentaro Noda, Yugo Tanaka, Norihisa Shigemura, Tomohiro Kawamura, Yinna Wang, Kosuke Masutani, et al.
Recent evidence suggests that molecular hydrogen has therapeutic value for dis-
ease states that involve inflammation. We hypothesized that drinking hydro-
gen-rich water (HW) daily would protect cardiac and aortic allograft recipients
from inflammation-associated deterioration. Heterotopic heart transplantation
with short-course tacrolimus immunosuppression and orthotopic aortic trans-
plantation w
Animal StudyHighlighted
MHID-09896642012Biochemical and Biophysical Research Communications
Yugo Tanaka, Norihisa Shigemura, Tomohiro Kawamura, Kentaro Noda, Kumiko Isse, Donna Beer Stolz, et al.
Background—We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen’s protective effects, we conducted a gene array analysis to identify changes in gene expression associated with hydrogen treatment.
Methods—Donor rats were exposed to mechanica
Animal StudyIn VitroObservational StudyHighlighted
MHID-5280800PMID: 226080092012Medical Gas Research
Mikako Ito, Masaaki Hirayama, Kazuaki Yamai, Sae Goto, Masafumi Ito, Masatoshi Ichihara, et al.
Background: Lactulose is a synthetic disaccharide that can be catalyzed only by intestinal bacteria in humans and rodents, and a large amount of hydrogen is produced by bacterial catalysis of lactulose. We previously reported marked effects of ad libitum administration of hydrogen water on prevention of a rat model of Parkinson’s disease (PD).
Methods: End-alveolar breath hydrogen concentrations
Literature Review
MHID-5039793PMID: 227201172012Oxidative Medicine and Cellular Longevity
Kinji Ohno, Mikako Ito, Masatoshi Ichihara, Masafumi Ito
Effects of molecular hydrogen on various diseases have been documented for 63 disease models and human diseases in the past four and a half years. Most studies have been performed on rodents including two models of Parkinson’s disease and three models of Alzheimer’s disease. Prominent effects are observed especially in oxidative stress-mediated diseases including neonatal cerebral hypoxia; Parkinson
Animal StudyHighlighted
MHID-6057293PMID: 227620402012International Journal of Ophthalmology
Feng M, Wang XH, Yang XB, Xiao Q, Jiang FG
·AIM: To explore the effect of saturated hydrogen saline on blue light-induced retinal damage in rats.
·METHODS: The retinal damage of rats was induced by blue light exposure for 6 hours and examined 8 hours, 16 hours and 24 hours after the exposure. One hundred female Sprague-Dawley rats were randomly divided into four groups. Group 1 included 30 rats received light exposure without any other tr
