Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells

Abstract

AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen -saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS -induced signaling proteins, including Myd88, IKK-茁, NF-kB, and PDCD4, were detected by Western blotting. RESULTS: The results demonstrated a marked down-regulation of miR -9 and miR -21 and up -regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-茁was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-kB expression. CONCLUSION: The results in the present study indicate that miR -9, miR -199 and miR -21 play an important role in the anti -inflammatory regulation of LPS -activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

Study details

Article type

Original Research

Disease / injury model

LPS-activated inflammation in retinal microglia (model for retinal inflammation/diabetic retinopathy)

Outcome

Neutral

Findings

Hydrogen treatment down-regulated miR-9 and miR-21, up-regulated miR-199, decreased Myd88 and IKK-β, increased PDCD4, and did not significantly change NF-κB expression in LPS-activated retinal microglia cells, suggesting an anti-inflammatory effect via miRNA and signaling pathway modulation.