An ethically guided preclinical device for phenotyping H2 production in laboratory rodents

Abstract

Background: Dihydrogen (H2) is produced endogenously by the intestinal microbiota through the fermentation of diet carbohydrates. Over the past few years, numerous studies have demonstrated the significant therapeutic potential of H2 in various pathophysiological contexts, making the characterization of its production in laboratory species of major preclinical importance. Methods: This study proposes an innovative solution to accurately monitor H2 production in free-moving rodents while respecting animal welfare standards. The developed device consisted of a wire rodent cage placed inside an airtight chamber in which the air quality was maintained, and the H2 concentration was continuously analyzed. After the airtightness and efficiency of the systems used to control and maintain air quality in the chamber were checked, tests were carried out on rats and mice with different metabolic phenotypes, over 12 min to 1-h experiments and repeatedly. H2 production rates (HPR) were obtained using an easy calculation algorithm based on a first-order moving average. Results: HPR in hyperphagic Zucker rats was found to be twice as high as in control Wistar rats, respectively, 2.64 and 1.27 nmol.s−1 per animal. In addition, the ingestion of inulin, a dietary fiber, stimulated H2 production in mice. HPRs were 0.46 nmol.s−1 for animals under control diet and 1.99 nmol.s−1 for animals under inulin diet. Conclusions: The proposed device coupled with our algorithm enables fine analysis of the metabolic phenotype of laboratory rats or mice with regard to their endogenous H2 production.

Study details

Article type

Original Research

Disease / injury model

Obesity/Diabetes (Zucker rats), Dietary modulation (inulin supplementation in mice)

Duration

41 weeks

Dose-dependent effect

No

Outcome

Positive

Findings

The study developed and validated a noninvasive, ethically guided device for continuous monitoring of endogenous hydrogen (H2) production in laboratory rodents. The device accurately distinguished metabolic phenotypes, showing that hyperphagic Zucker rats had twice the H2 production rate of control Wistar rats, and inulin supplementation significantly increased H2 production in mice. The device enables sensitive, real-time phenotyping of H2 production in both individual and groups of rodents.