Effect of molecular hydrogen, a novelly-established antioxidant, on the retinal degeneration of hereditary retinitis pigmentosa: an in-vivo study

Abstract

Objective Our research was performed in order to explore the effects of molecular hydrogen (H2), a novely-established antioxidant, on the retinal degeneration in rd1 mice, an animal model of inherited retinitis pigmentosa (RP). Methods The rd1 mice were divided randomly into control and H2 intervention groups. Mice from other groups received H2 intervention in three modes, two modes of the hydrogen gas (HG) and one model of hydrogen-rich saline (HRS). At 14 days post born (P14) and P21, various indicators were detected in all mice, including eletroretinogram (ERG), fundus phography, optical coherence tomography (OCT), and retinal immunostaining of microglia cells’ marker, Iba1. Results The ERG amplitude in mice from the control and H2 intervention groups showed no statistical differences (p > 0.05). At P14 and P21, no significant difference in the distance from the retinal pigment epithelium to the outer plexiform layer on OCT from mice of the above two groups was found (p > 0.05). The thickness of the outer nuclear layer (ONL) in mice at P14 and P21 showed no statistical differences between the control group and the H2 intervention group (p > 0.05). In the aspect of the number of Iba1-positive cells, we did not found any significant differences between the two groups (p > 0.05). Conclusion Different forms of H2 intervention (hydrogen-rich saline and hydrogen gas) had no obvious effects on the course of retinal degeneration in rd1 mice. The specific mechanism of photoreceptor degeneration in the hereditary RP mouse model may be different, requiring different medical interventions.

Study details

Article type

Original Research

Disease / injury model

Retinitis pigmentosa (rd1 mice)

Duration

21 days

Dose comparison

Only a single hydrogen concentration was used for each method; no dose/concentration comparison was performed.

Dose-dependent effect

No

Outcome

Mixed

Findings

Different forms of molecular hydrogen intervention (hydrogen-rich saline and hydrogen gas) had no significant effect on the course of retinal degeneration in rd1 mice, an inherited model of retinitis pigmentosa. No improvements were observed in retinal function, morphology, or microglial activation compared to controls.