Anti-obesity Effect of Alkaline Reduced Water in High Fat-Fed Obese Mice

Abstract

Whether or not alkaline reduced water (ARW) has a positive effect on obesity is unclear. This study aims to prove the positive effect of ARW in high-fat (HF) diet-induced obesity (DIO) in C57BL/6 mice model. Toward this, obesity was induced by feeding the C57BL/6 male mice with high-fat diet (w/w 45% fat) for 12 weeks. Thereafter, the animals were administered with either ARW or tap water. Next, the degree of adiposity and DIO-associated parameters were assessed: clinico-pathological parameters, biochemical measurements, histopathological analysis of liver, the expression of cholesterol metabolism-related genes in the liver, and serum levels of adipokine and cytokine. We found that ARW-fed mice significantly ameliorated adiposity: controlled body weight gain, reduced the accumulation of epididymal fats and decreased liver fats as compared to control mice. Accordingly, ARW coordinated the level of adiponectin and leptin. Further, mRNA expression of cytochrome P450 (CYP)7A1 was upregulated. In summary, our data shows that ARW intake inhibits the progression of HF-DIO in mice. This is the first note on anti-obesity effect of ARW, clinically implying the safer fluid remedy for obesity control.

Study details

Article type

Original Research

Disease / injury model

High-fat diet-induced obesity (DIO) in mice

Duration

12 weeks

Dose-dependent effect

No

Outcome

Positive

Findings

Alkaline reduced water (ARW) intake significantly inhibited the progression of high-fat diet-induced obesity in mice by reducing body weight gain, epididymal fat accumulation, and liver fat, while also modulating adipokine and cytokine levels and upregulating hepatic CYP7A1 expression.